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Laboratory Management |
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Budgets (Research grants) Program and Project Management - Grantsmanship Research Design (planning, execution & publication) Research Communication (articles, reviews, book chapters, books) Team Building (Teaching & research groups) Training and mentoring staff and students (Undergraduate, graduate, Master & PhD) |
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Knowledge Areas |
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Cell & Molecular Biology (with focus on tumor biology) Biochemistry Oncology Pharmacology Preclinical Anticancer Drug Screening Cancer Stem Cells |
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Technical Skills |
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Cell Biology
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Flow cytometry / Cell cycle analysis / Cell and tissue culture / Cell death analysis (apoptosis, autophagy) / Short term proliferation assay (e.g. CCK) / Long-term proliferation assay / Senescence assay (β-galactosidase) / Cell migration assay
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Molecular Biology
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DNA (plasmid and genomic) and RNA purification / Cloning and site-directed mutagenesis / PCR, RT-PCR, single cell PCR, qPCR arrays / Northern and Southern blot / Chromatin immunoprecipitation / Pulse field electrophoresis / Biolistic transformation
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Proteins
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Purification, immunoprecipitation Western Blot 2D-PAGE
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Lipids
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Extraction/ purification / TLC
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Biochemistry
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Enzymatic activity (topoisomerase I and II activity, protein kinase activity)
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Microscopy
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Fluorescence, Light / Histo and cytochemistry /Confocal Microscopy
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Animal models
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Cancer (RG2 rat glioma model) / Diabetes (Streptozotocin)
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Miscellaneous
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Radiolabelling (protein, lipids, DNA, posttranslational modifications: phosphorylation, glycosylation) |
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