MOLECULAR BIOLOGY: Choose a subject ... SYLLABUS Lecture 1: MOLECULAR BIOLOGY: ENZYMES AND METABOLISM Lecture 2: A. Enzyme Structure and Function Lecture 3: 1. Function of enzymes in catalyzing biological reactions Lecture 4: 2. Reduction of activation energy Lecture 5: 3. Substrates and enzyme specificity Lecture 6: B. Control of Enzyme Activity Lecture 7: 1. Feedback inhibition Lecture 8: 2. Competitive inhibition Lecture 9: 3. Noncompetitive inhibition Lecture 10: C. Basic Metabolism Lecture 11: 1. Glycolysis (anaerobic and aerobic, substrates and products) Lecture 12: 2. Krebs cycle (substrates and products, general features of the pathway) Lecture 13: 3. Electron transport chain and oxidative phosphorylation (substrates and products, general features of the pathway) Lecture 14: 4. Metabolism of fats and proteins Lecture 15: MOLECULAR BIOLOGY: DNA AND PROTEIN SYNTHESIS DNA Structure and Function Lecture 16: A. DNA Structure and Function Lecture 17: 1. Double-helix structure Lecture 18: 2. DNA composition (purine and pyrimidine bases, deoxyribose, phosphate) Lecture 19: 3. Base-pairing specificity, concept of complementarity Lecture 20: 4. Function in transmission of genetic information Lecture 21: B. DNA Replication Lecture 22: 1. Mechanism of replication (separation of strands, specific coupling of free nucleic acids, DNA polymerase, primer required) Lecture 23: 2. Semiconservative nature of replication Lecture 24: C. Repair of DNA Lecture 25: 1. Repair during replication Lecture 26: 2. Repair of mutations Lecture 27: D. Recombinant DNA Techniques Lecture 28: 1. Restriction enzymes Lecture 29: 2. Hybridization Lecture 30: 3. Gene cloning Lecture 31: 4. PCR Lecture 32: PROTEIN SYNTHESIS Lecture 33: A. Genetic Code Lecture 34: 1. Typical information flow (DNA → RNA → protein) Lecture 35: 2. Codon-anticodon relationship, degenerate code Lecture 36: 3. Missense and nonsense codons Lecture 37: 4. Initiation and termination codons (function, codon sequences) Lecture 38: B. Transcription Lecture 39: 1. mRNA composition and structure (RNA nucleotides, 5′ cap, poly-A tail) Lecture 40: 2. tRNA and rRNA composition and structure (e.g., RNA nucleotides) Lecture 41: 3. Mechanism of transcription (RNA polymerase, promoters, primer not required) Lecture 42: C. Translation Lecture 43: 1. Roles of mRNA, tRNA, and rRNA; RNA base-pairing specificity Lecture 44: 2. Role and structure of ribosomes Lecture 45: MOLECULAR BIOLOGY: EUKARYOTES Lecture 46: A. Eukaryotic Chromosome Organization Lecture 47: 1. Chromosomal proteins Lecture 48: 2. Telomeres, centromeres Lecture 49: B. Control of Gene Expression in Eukaryotes Lecture 50: 1. Transcription regulation Lecture 51: 2. DNA binding proteins, transcription factors Lecture 52: 3. Cancer as a failure of normal cellular controls, oncogenes, tumor suppressor genes Lecture 53: 4. Posttranscriptional control, basic concept of splicing (introns, exons)